Gold dredge nozzle 5' hydro force nozzle1/31/2024 A pump integrated with a micronozzle array was used to transport single HeLa cells into nozzle holes. The flow was bidirectionally controlled by changing the DC bias. Pt/Ti and indium tin oxide (ITO) electrodes were used to study the effect of DC bias and peak-to-peak voltage and electrodes in a low conductivity isotonic solution. This paper reports on the integration of a polydimethylsiloxane (PDMS) micronozzle array and bidirectional electrokinetic pumps driven by DC-biased AC voltages. If one-side-open multi-channel microdevices are integrated with micropumps, the devices will achieve higher throughput in the manipulation of single cells while maintaining flexibility and open accessibility. High throughput reconstruction of in vivo cellular environments allows for efficient investigation of cellular functions.
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